TH1/TH2 pattern of renal tissues in acute and chronic animal models treated with mesenchymal stem cells

Autores/as

  • Patricia Semedo Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
  • Carolina Gardini Palasio Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
  • Cassiano Donizetti Oliveira Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
  • Carla Quarim Feitoza Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
  • Giselle Martins Gonçalves Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
  • Pamella Mei Wang Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
  • Marcos Antonio Cenedeze Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
  • Alvaro Pacheco-Silva Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
  • Vicente de Paula Teixeira General Pathology Division, Triangulo Mineiro Medical School, Uberaba, Minas Gerais, Brazil.
  • Marlene Antônia dos Reis General Pathology Division, Triangulo Mineiro Medical School, Uberaba, Minas Gerais, Brazil.
  • Niels Olsen Saraiva Câmara Laboratório de Imunologia Clínica e Experimental. Division of Nephrology. Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil /Laboratory of Transplantation Immunology. Department of Immunology, Universidade de São Paulo, São Paulo, Brazil.

DOI:

https://doi.org/10.53855/bjt.v10i2.330

Palabras clave:

Mesenchymal Stem Cell Transplantation, Renal Insufficiency, Acute, Renal Insufficiency, Chronic

Resumen

Mesenchymal stem cell (MSC) therapy is a promising new therapy for kidney diseases. Many authors have been demonstrated that the MSC treatment leads to N improvement of the renal function in acute damaged models. However, the way it works still remains elusive. Purpose: To evaluate whether the renal protection provided by MSC administration seen in acute and chronic renal remnant damaged models involves modulation of the inflammation. Methods: MSC were attained from bone marrow of male Wistar rats. Female Wistar were subjected to ischemia-reperfusion damage by clamping renal pedicles for 1 hour. After a 6h reperfusion, 2.105 MSC were administrated i.v. in the chronic model. The nephrectomy models 5/6 were treated with 1.106 MSC administered i.v. after two weeks. Results: After a 24h reperfusion, MSC-treated animals presented a significant improvement of the renal function associated to decreased levels of IL-1β, IL-6 and TNF-α gene expression. Interestingly, the IL-4 mRNA expression increased in parallel. Furthermore, a higher expression of PCNA and an increase in the Bcl2/Bad ratio were seen in kidney tissues of MSC-treated animals. In the remnant model, this modulation in the cytokine pattern was sustained, despite a lack of amelioration of the renal dysfunction. Moreover, the expressions of TGF-β, MMP-9, TIMP-1 and Smad7 were not altered, although PAI-1 was significantly decreased. Conclusion: MSC therapy can indeed modulate the inflammatory response following acute and chronic renal injuries, accelerating the tubular repair; however, long-term outcomes seem not to be halted by this therapy.

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Publicado

2007-06-01

Cómo citar

Semedo, P., Palasio, C. G., Oliveira, C. D., Feitoza, C. Q., Gonçalves, G. M., Wang, P. M., Cenedeze, M. A., Pacheco-Silva, A., Teixeira, V. de P., Reis, M. A. dos, & Câmara, N. O. S. (2007). TH1/TH2 pattern of renal tissues in acute and chronic animal models treated with mesenchymal stem cells. Brazilian Journal of Transplantation, 10(2), 708–716. https://doi.org/10.53855/bjt.v10i2.330

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