Bioprinting in Organ Transplantation: From Experimental Models to Clinical Prospects
Keywords:
Tissue Engineering, 3D Printing Technologies, Graft Viability, Biomedical Innovation, Implantable ConstructsAbstract
Background: Bioprinting has emerged as an innovative technology in organ transplantation and regenerative medicine, aiming to address pressing challenges such as the shortage of donor organs and the need for effective tissue repair. By leveraging advanced 3D printing techniques, bioprinting enables the fabrication of functional tissues and organs with precise architectural and biological properties. Methods: This review provides an in-depth analysis of the latest advancements in bioprinting, focusing on cutting-edge techniques, the development of bioinks, and their applications in tissue engineering. It examines significant breakthroughs in the creation of vascularized and transplantable organ prototypes and explores the role of bioprinting in personalized medicine. Results: The findings highlight the transformative impact of bioprinting in the biomedical field, particularly in drug testing, therapeutic modeling, and patient-specific treatment strategies. Additionally, key challenges—including technological limitations, ethical concerns, and regulatory considerations—are discussed to provide a comprehensive understanding of the field’s progress and potential obstacles. Conclusion: Bioprinting holds immense promise for revolutionizing global healthcare by offering solutions for organ shortages and advancing regenerative medicine. However, continued research and innovation are necessary to overcome existing challenges and facilitate its clinical translation into mainstream medical practice.
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References
1. Persaud A, Maus A, Strait L, Zhu D. 3D Bioprinting with Live Cells. Eng Regen 2022;3(3):292-309. https://doi.org/10.1016/j.engreg.2022.07.002
2. Gu Z, Fu J, Lin H, He Y. Development of 3D bioprinting: From printing methods to biomedical applications. Asian J Pharm Sci 2019;15(5):529-57. https://doi.org/10.1016/j.ajps.2019.11.003
3. Atala A, Bauer SB, Soker S, Yoo JJ, Retik AB. Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet 2006;367(9518):1241-6. https://doi.org/10.1016/S0140-6736(06)68438-9
4. Mierke CT. Bioprinting of cells, organoids and organs-on-a-chip together with hydrogels improves structural and mechanical cues. Cells 2024;13(19):1638. https://doi.org/10.3390/cells13191638
5. Jongh D, Massey EK, Cronin AJ, Schermer MHN, Bunnik EM, VANGUARD Consortium. Early-phase clinical trials of bio-artificial organ technology: A systematic review of ethical issues. Transpl Int 2022;35:10751. https://doi.org/10.3389/ti.2022.10751
6. Fang Y, Guo Y, Liu T, Xu R, Mao S, Mo X, et al. Advances in 3D bioprinting. Chin J Mech Eng 2022;1(1):100011. https://doi.org/10.1016/j.cjmeam.2022.100011
7. Budharaju H, Sundaramurthi D, Sethuraman S. Embedded 3D bioprinting – An emerging strategy to fabricate biomimetic & large vascularized tissue constructs. Bioact Mater 2023;32:356-84. https://doi.org/10.1016/j.bioactmat.2023.10.012
8. Chen X, Anvari-Yazdi AF, Duan X, Zimmerling A, Gharraei R, Sharma N, et al. Biomaterials / bioinks and extrusion bioprinting. Bioactive Materials 2023;28:511-36. https://doi.org/10.1016/j.bioactmat.2023.06.006
9. Brahme P, Rarokar N, Kumbhalkar R, Saoji S, Khedekar P. Natural and synthetic polymeric hydrogel: a bioink for 3D bioprinting of tissue models. J Drug Deliv Sci Technol 2024(Part A);106204. https://doi.org/10.1016/j.jddst.2024.106204
10. Do AV, Khorsand B, Geary SM, Salem AK. 3D Printing of Scaffolds for Tissue Regeneration Applications. Adv Healthc Mater 2015;4(12):1742-62. https://doi.org/10.1002/adhm.201500168
11. Selim M, Mousa HM, Abdel-Jaber G, Barhoum A, Abdal-Hay A. Innovative designs of 3D scaffolds for bone tissue regeneration: Understanding principles and addressing challenges. Eur Polym J 2024;215:113251. https://doi.org/10.1016/j.eurpolymj.2024.113251
12. Mirshafiei M, Rashedi H, Yazdian F, Rahdar A, Baino F. Advancements in tissue and organ 3D bioprinting: Current techniques, applications, and future perspectives. Mater Des 2024;240:112853. https://doi.org/10.1016/j.matdes.2024.112853
13. Datta P, Ayan B, Ozbolat IT. Bioprinting for vascular and vascularized tissue biofabrication. Acta Biomater 2017;51:1-20. https://doi.org/10.1016/j.actbio.2017.01.035
14. Wang X, Zhang D, Singh YP, Yeo M, Deng G, Lai J, et al. progress in organ bioprinting for regenerative medicine. Engineering 2024;42:121-42. https://doi.org/10.1016/j.eng.2024.04.023
15. Cui H, Miao S, Esworthy T, Zhou X, Lee SJ, Liu C, et al. 3D bioprinting for cardiovascular regeneration and pharmacology. Adv Drug Deliv Rev 2018;132:252-69. https://doi.org/10.1016/j.addr.2018.07.014
16. Yeo M, Sarkar A, Singh YP, Derman ID, Datta P, Ozbolat IT. Synergistic coupling between 3D bioprinting and vascularization strategies. Biofabrication 2023;16(1):012003. https://doi.org/10.1088/1758-5090/ad0b3f
17. Sexton ZA, Hudson AR, Herrmann JE, Shiwarski DJ, Pham J, Szafron JM, et al. Rapid model-guided design of organ-scale synthetic vasculature for biomanufacturing. ArXiv 2023;arXiv:2308.07586v1. https://doi.org/10.48550/arXiv.2308.07586
18. Tripathi S, Mandal SS, Bauri S, Maiti P. 3D bioprinting and its innovative approach for biomedical applications. MedComm 2022;4(1):e194. https://doi.org/10.1002/mco2.194
19. Chiticaru EA, Ioniță M. Commercially available bioinks and state-of-the-art lab-made formulations for bone tissue engineering: a comprehensive review. Mater Today Bio 2024;29:101341. https://doi.org/10.1016/j.mtbio.2024.101341
20. Zhou J, Li Q, Tian Z, Yao Q, Zhang M. Recent advances in 3D bioprinted cartilage-mimicking constructs for applications in tissue engineering. Mater Today Bio 2023;23:100870. https://doi.org/10.1016/j.mtbio.2023.100870
21. Varkey M, Visscher DO, van Zuijlen PPM, Atala A, Yoo JJ. Skin bioprinting: the future of burn wound reconstruction? Burns Trauma 2019;7:4. https://doi.org/10.1186/s41038-019-0142-7
22. Kolimi P, Narala S, Nyavanandi D, Youssef AAA, Dudhipala N. Innovative treatment strategies to accelerate wound healing: trajectory and recent advancements. Cells 2022;11(15):2439. https://doi.org/10.3390/cells11152439
23. Huang G, Zhao Y, Chen D, Wei L, Hu Z, Li J, et al. Applications, advancements, and challenges of 3D bioprinting in organ transplantation. Biomater Sci 2024;12(6):1425-44. https://doi.org/10.1039/d3bm01934a
24. Mathur V, Agarwal P, Kasturi M, Srinivasan V, Seetharam RN, Vasanthan KS. Innovative bioinks for 3D bioprinting: Exploring technological potential and regulatory challenges. J Tissue Eng 2025;16:1-31. https://doi.org/10.1177/20417314241308022
25. Mota C, Camarero-Espinosa S, Baker MB, Wieringa P, Moroni L. Bioprinting: from tissue and organ development to in vitro models. Chem Rev 2020;120(19):10547-607. https://doi.org/10.1021/acs.chemrev.9b00789
26. Wang Z, Wang L, Li T, Liu S, Guo B, Huang W, Wu Y. 3D bioprinting in cardiac tissue engineering. Theranostics 2021;11(16):7948-69. https://doi.org/10.7150/thno.61621
27. Yaneva A, Shopova D, Bakova D, Mihaylova A, Kasnakova P, Hristozova M, et al. The progress in bioprinting and its potential impact on health-related quality of life. Bioengineering 2023;10(8):910. https://doi.org/10.3390/bioengineering10080910
28. Shopova D, Yaneva A, Bakova D, Mihaylova A, Kasnakova P, Hristozova M, et al. (Bio)printing in personalized medicineopportunities and potential benefits. Bioengineering 2023;10(3):287. https://doi.org/10.3390/bioengineering10030287
29. Lam EHY, Yu F, Zhu S, Wang Z. 3D bioprinting for next-generation personalized medicine. Int J Mol Sci 2023;24(7):6357. https://doi.org/10.3390/ijms24076357
30. Parihar A, Parihar DS, Gaur K, Arya N, Choubey VK, Khan R. 3D bioprinting for drug development and screening: recent trends towards personalized medicine. Hybrid Adv 2024;100320. https://doi.org/10.1016/j.hybadv.2024.100320
31. Yang K, Wang L, Vijayavenkataraman S, Yuan Y, Tan E, Kang L. Recent applications of three dimensional bioprinting in drug discovery and development. Adv Drug Deliv Rev 2024;115456. https://doi.org/10.1016/j.addr.2024.115456
32. Ricci G, Gibelli F, Sirignano A. Three-dimensional bioprinting of human organs and tissues: bioethical and medicolegal implications examined through a scoping review. Bioengineering 2023;10(9):1052. https://doi.org/10.3390/bioengineering10091052
33. Kantaros A, Ganetsos T, Petrescu FIT, Alysandratou E. Bioprinting and intellectual property: challenges, opportunities, and the road ahead. Bioengineering 2025;12(1):76. https://doi.org/10.3390/bioengineering12010076
34. Vijayavenkataraman S. 3D bioprinting: challenges in commercialization and clinical translation. J 3D Print Med 2023;7(2):3DP8. https://doi.org/10.2217/3dp-2022-0026
35. Xu HQ, Liu JC, Zhang ZY, Xu CX. A review on cell damage, viability, and functionality during 3D bioprinting. Mil Med Res 2022;9(1):70. https://doi.org/10.1186/s40779-022-00429-5
36. Derman ID, Moses JC, Rivera T, Ozbolat IT. Understanding the cellular dynamics, engineering perspectives and translation prospects in bioprinting epithelial tissues. Bioact Mater 2024;43:195-224. https://doi.org/10.1016/j.bioactmat.2024.09.025
37. Hasan MM, Ahmad A, Akter MZ, Choi YJ, Yi HG. Bioinks for bioprinting using plant-derived biomaterials. Biofabrication 2024;16(4):042004. https://doi.org/10.1088/1758-5090/ad6932
38. Mladenovska T, Choong PF, Wallace GG, O’Connell CD. The regulatory challenge of 3D bioprinting. Regen Med 2023;18(8):659-74. https://doi.org/10.2217/rme-2022-0194
39. Tong A, Pham QL, Abatemarco P, Mathew A, Gupta D, Iyer S, et al. Review of low-cost 3D bioprinters: state of the market and observed future trends. SLAS Technol 2021;26(4):333-66. https://doi.org/10.1177/24726303211020297
40. Kirillova A, Bushev S, Abubakirov A, Sukikh G. Bioethical and legal issues in 3D bioprinting. Int J Bioprint 2020;6(3):272. https://doi.org/10.18063/ijb.v6i3.272
41. Gilbert F, O’Connell CD, Mladenovska T, Dodds S. Print me an organ? Ethical and regulatory issues emerging from 3D bioprinting in medicine. Sci Eng Ethics 2017;24(1):73–91. https://doi.org/10.1007/s11948-017-9874-6
42. Devarapalli P, Ajay D. The impact of 3D bioprinting innovation on IP ecosystem and patent law: an Indian and US perspective. In: Singh K, Chongtham N, Trikha R, Bhardwaj M, Kaur S, editors. Science, technology and innovation ecosystem: an Indian and global perspective. 2024. p. 197-210. https://doi.org/10.1007/978-981-97-2815-2_9
43. Vijayavenkataraman S, Lu W, Fuh J. 3D bioprinting – an ethical, legal and social aspects (ELSA) framework. Bioprinting 2016;1-2:11–21. https://doi.org/10.1016/j.bprint.2016.08.001
44. Zhang Z, Zhou X, Fang Y, Xiong Z, Zhang T. AI-driven 3D bioprinting for regenerative medicine: from bench to bedside. Bioact Mater 2024;45:201-30. https://doi.org/10.1016/j.bioactmat.2024.11.021
45. Barjuei ES, Shin J, Kim K, Lee J. Precision improvement of robotic bioprinting via vision-based tool path compensation. Sci Rep 2024;14:17764. https://doi.org/10.1038/s41598-024-68597-z
46. Aljohani W, Ullah MW, Zhang X, Yang G. Bioprinting and its applications in tissue engineering and regenerative medicine. Int J Biol Macromol 2017;107:261-75. https://doi.org/10.1016/j.ijbiomac.2017.08.171
47. Jain P, Kathuria H, Dubey N. Advances in 3D bioprinting of tissues/organs for regenerative medicine and in-vitro models. Biomaterials 2022;287:121639. https://doi.org/10.1016/j.biomaterials.2022.121639
48. Jovic TH, Combellack EJ, Jessop ZM, Whitaker IS. 3D bioprinting and the future of surgery. Front Surg 2020;7:609836. https://doi.org/10.3389/fsurg.2020.609836
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